Skal Labissiere made the most of his first start since Dec. 12, chipping in 11 points and eight rebounds for the Wildcats.
Kentucky had no answer inside for John Egbunu, who finished with nine dunks and a career-high 27 points, but the Cats more than made up for it by hitting 7 of 10 shots from 3-point range.
They hit all five shots from behind the arc in the decisive second half.
The Wildcats (22-8, 12-5 Southeastern Conference) squandered a 14-point lead late in the first half, and Florida (17-13, 8-9) could have been in front at the break if not for missing 10 free throws in the first 20 minutes. The Gators ended up 16 of 37 from the line.